The Perceived Stress Scale (PSS-10) assesses whether individuals perceive their lives as stressful, uncontrollable, and unpredictable. Although perceived stress is a relatively stable characteristic, significant variability exists across individuals, suggesting that PSS-10 scores reflect the interaction between life experiences and an individual's ability to manage stress. Difficulties in emotion regulation may shape one's perceptions of their ability to handle stress. Emotion regulation strategies, such as emotion differentiation and cognitive reappraisal, influence responses to stress and moderate the relationship between perceived stress and internalizing psychopathologies. Likewise, resilience, which represents a person's ability to cope with perceived stressors, inversely correlates with perceived stress. We examined the moderating role of resilience on the relationship between difficulties in emotion regulation and perceived stress using the Difficulties in Emotion Regulation Scale (DERS), the PSS-10, and the Connor-Davidson Resilience Scale (CD-RISC). Twenty graduate/16 undergraduate students from two U.S. universities wore an Oura ring for 40 days to gather biometric data and assess the bidirectionality of physiological and emotional arousal. Participants completed DERS prior to participation via a smartphone application utilized for Experience Sampling Methodology data collection. A linear mixed model analysis revealed that difficulties in emotion regulation significantly predicted perceived stress (β=0.18, p<0.0001), with higher DERS scores associated with increased perceived stress. Time had a significant effect on PSS (β=0.17, p<0.0001), indicating that perceived stress increased throughout the study. Resilience did not significantly moderate this relationship, nor was there a significant three-way interaction with time. Resilience showed a significant inverse relationship with perceived stress (β=-1.12, p=0.05). While the DERS and CD-RISC independently predict stress, their interaction does not reach significance, suggesting that resilience and emotion regulation contribute independently to stress levels rather than one moderating the effect of the other. Higher resilience appears protective, significantly predicting lower perceived stress, even when accounting for emotion regulation difficulties.