Self-care practices are crucial for promoting well-being and are particularly relevant for workers in the human services sector, helping them manage work-related stress and maintain their occupational health. However, there is a lack of psychometrically sound instruments to measure self-care practices among workers. We aimed to assess the psychometric properties of the Self-Care Practices Scale (SCPS) for Portuguese workers and explore its association with well-being and burnout. Two related studies were conducted. Study 1 reports the adaptation and the psychometric assessment of the SCPS with a sample of 654 Portuguese employees (74.2% female, M = 47.29 years, SD = 11.04). Factor analysis indicated that a bi-factorial model provided a better fit to the data, with an 18-item general factor and two 9-item factors assessing personal and professional self-care practices. The scale offers adequate evidence of reliability. Study 2 assesses the adequacy of SCPS in a sample of educators (N = 657, 75.5% female, M = 51.43 years, SD = 8.78) and examines the relationships between teachers' self-care practices and occupational health outcomes. Findings supported the structural validity of the instrument's bi-factorial solution and adequate reliability. Multi-group invariance was attained across gender, work context, and instructional level. Results also supported the SCPS's discriminant and criterion validities against external measures. Independent samples t-test revealed that teachers engaged in fewer self-care practices than workers in non-educational contexts, particularly in terms of professional self-care practices. Multiple hierarchical regression analyses supported the predictive role of teachers' self-care practices on their personal well-being, work engagement, and burnout. Findings help answer relevant questions about the role of self-care in the occupational health and well-being of teachers, contributing to an evidence-based discussion on the importance of promoting self-care practices in work settings.