Biodiversity loss, a largely anthropogenically driven phenomenon, represents a monumental challenge for both the natural and human world that has serious repercussions. Animal Ambassadors are employed by zoological parks, ecotourism, and by flagship species promotional programmes to combat biodiversity loss by conveying conservation messaging to increase the publics' pro-environmental attitudes. A scoping review was undertaken to address the animal ambassador effect on human pro-environmental attitudes and behaviours, following the PRISMA-ScR guidelines. A search was conducted using the databases Medline, PsycInfo, Scopus and Web of Science, and a grey literature search was undertaken using the ProQuest Dissertation and Theses Global Database, along with the search engine Google (advanced search) and Google Scholar (advanced search). The results yielded 46 articles that met the review criteria, with the majority of articles indicating positive effects on human pro-environmental attitudes and behaviours. This included non-living AA programmes (such as VR or AA mascots), which have implications for future AA interactions as steps can be taken to potentially lessen the amount of live AA programmes that can have negative impacts on the animal's welfare, and still promote pro-environmental attitudes and behaviours.