Efforts to enhance quality of work life or quality of work-related life have progressively gained support in recent decades. Among the major emphasis of positive psychology in the workplace is the design of job features to enhance employees' quality of life such as well-being and happiness. Hence, the features of job in terms of job characteristics are necessary for the realization of these goals of positive psychology. There has been much research on job characteristics, employee well-being and happiness at work, but there has been little effort to study these issues among female teachers in the non-Western context of Nigeria. In response, this correlational study examines job characteristics, employee well-being and happiness in 146 female teachers from 26 to 55 year (M = 34.60) drawn from female secondary school teachers in Enugu metropolis, South-eastern Nigeria. The participants completed measures of job characteristics, employee well-being and happiness. The results of the correlations show that employee well-being has a positive relationship with happiness as well as skill variety, task identity, task significance and feedback dimensions of job characteristics. Happiness has positive relationships with skill variety, task identity and feedback. Results of the hierarchical multiple regression show that employee well-being and skill variety positively predict happiness, while task significance and autonomy negatively predict happiness. Skill variety and task identity moderate the relationship between employee well-being and happiness. These findings have implications for work design, positive psychology research, policies and practice that enhance teachers' happiness, well-being, professional success and personal fulfilment. The results also provide evidence foundation for policy makers, educational psychologists, industrial-organizational psychologists, clinicians and other allied health professionals to inform their training and support programmes necessary for enhancing general well-being in the workplace.