The aim of the study was to investigate sex- and temperament-related differences in meaning attribution. This study applied Osgood's semantic differential using the most neutral and abstract concepts and basic adjectives bipolar scales (as a semi-projective method to study the characteristics of meaning attribution). The study was conducted in English for 1180 Canadians, in Russian for 167 Russians and in Chinese 161 Chinese subjects. The results showed gender and temperament-related differences in meaning attribution. Males in all three samples attributed more negative estimations to abstract objects than females. People with stronger endurance and tempo in verbal-social activities differed dramatically from other temperament groups. At the same time there were cultural differences in semantic spaces of various gender and temperament groups. Gender and temperament differences in meaning attribution indicate a possible contribution of biological factors in resulting attitudes. The differences between "extraverts" and other temperament groups also show deficits of so-called "lexical approach" in studying personality and individual differences, which rely on factor analysis of descriptors of human behavior.