Organ transplantation, the process of replacing a failing organ with one from a voluntary donor, has primarily focused on recipients, leading to a gap in research concerning donors' psychological well-being. Existing studies suggest living organ donors may experience post-operative stress, depression, and anxiety. This study aimed to address this by the psychological impact of organ donation surgery on donors. We compared the pre- and post-test psychological health of actual donors with a control group of potential transplant volunteers who had not undergone surgery. A 2 (pre-test/post-test) x 2 (control group/actual donor group) mixed-design repeated measures ANOVA was conducted for depression, anxiety, and stress levels, separately. The findings revealed a statistically significant increase in "stress" levels from pre-test (M=.57, SD=.46) to post-test (M=.72, SD=.54) [F(1, 90)=5.586, p=.020]. Moreover, groups differed significantly in pre-test stress scores [F(1, 90)=9.267, p=.003], with the actual donor group (M=.44, SD=.38) indicating significantly lower preoperative stress compared to the control group (M=.75, SD=.51) (t=-3.337, p<.001). For "depression", pre-test scores showed the actual donor group (M=.38, SD=.33) had significantly lower depression than the control group (M=.64, SD=.49) (t=-2.96, p=.004). A significant time by group interaction effect for depression [F(1, 90)=7.072, p=.009, ηp²=.073] suggested differing depression trajectories between groups over time. "Anxiety" levels significantly increased from pre-test (M=.39, SD=.40) to post-test (M=.61, SD=.54) across all participants [F(1, 90)=12.983, p=.001]. Preoperative anxiety levels were significantly lower in the actual donor group (M=.30, SD=.35) compared to the control group (M=.50, SD=.43) (t=-2.441, p=.017). A significant time by group interaction effect was also observed for anxiety scores [F(1, 90)=10.455, p=.002, ηp²=.104]. Although actual living organ donors exhibit lower psychological symptoms prior to surgery, it is obvious that the surgical process is exhausting for them and that their psychological symptoms increase to a certain extent.