Burnout has become one of the most significant psychosocial risks in modern workplaces, adversely impacting employees' well-being and job satisfaction. Perceived organizational support (POS), the extent to which employees feel valued and supported by their organization, is recognized as a crucial protective factor against burnout. This study aims to explore the relationship between burnout and job satisfaction while investigating the potential moderating role of POS. A total of 288 employees from various occupational sectors in Portugal participated by completing an online questionnaire. The survey included validated measures for burnout (using the Copenhagen Burnout Inventory), job satisfaction (via the Short Index of Job Satisfaction), and perceived organizational support (using the colleague and supervisor support subscales of the COPSOQ III). The results showed that burnout is negatively related to job satisfaction, indicating that higher levels of exhaustion are associated with lower satisfaction at work. In contrast, perceived organizational support (POS) is positively correlated with job satisfaction and negatively correlated with burnout, with supervisor support being particularly significant. The hypothesized role of perceived organizational support (POS) as a moderator in the relationship between burnout and job satisfaction was not confirmed. These findings highlight that while POS directly contributes to increased job satisfaction and reduced burnout, it may not be enough to mitigate the negative effects of burnout on its own. The study emphasizes the importance of organizations enhancing their support structures and implementing additional strategies—such as workload management, leadership training, and well-being programs—to create healthier and more sustainable workplaces.